Journal: Food Chemistry: X

Article Title: Major anthocyanins in elderberry effectively trap methylglyoxal and reduce cytotoxicity of methylglyoxal in HepG2 cell line

doi: 10.1016/j.fochx.2022.100468

Figure Lengend Snippet: Effect of methylglyoxal (MGO) and elderberry extracts using cell-models: Dose-dependent effect of exposure to MGO, for 24 h and 48 h, on Caco-2 cell viability (a1) and HepG2 cell viability (a3) and cell morphology recorded after 48 h of exposure to MGO (a2 and a4, Caco-2 and HepG2 cells, respectively), photos captured using an ocular camera (ODC-89 camera, Kern & Sohn) coupled to a microscope (amplification 100x). (Significant differences between samples and control cells ( p < 0.05) are denoted by *and #, for 24 h and 48 h of exposure to MGO, respectively). b) Evaluation of cytoprotective effect promoted by pre- or co-incubation of Caco-2 cells with elderberry extracts [(elderberry extract (E), rich-phenolic extract (M), and non-phenolic extract (H)], from Sabugueiro (So), Sabugueira (Sa) and Bastardeira (Ba) cultivars, against 3 mM MGO. c) Evaluation of cytoprotective effect promoted by pre- and co-incubation of HepG2 cells with elderberry extracts against [(elderberry extract (E) and rich-phenolic extract (M)], from Sabugueiro (So), Sabugueira (Sa) and Bastardeira (Ba) cultivars, against 2 mM MGO. In b) and c), statistical significance ( p < 0.05) between control cells and samples is denoted as *; the # compares extracts with the MGO control at the respective condition; and * over a square bracket compares pre- and co-incubation at the same concentration.

Article Snippet: Caco-2 (human colon adenocarcinoma, CLS, Eppelheim, Germany) and HepG2 (human hepatoma cell line; ATCC, Rockville, MD, USA) cells were maintained in DMEM (Dulbecco’s Modified Eagle Medium) containing 25 mM glucose supplemented with 10 % (v/v) fetal bovine serum (FBS; Gibco, Life technologies), 2 mM l -glutamine (Gibco, Life Technologies), 100 U/mL penicillin (Life Technologies) and 100 μg/mL streptomycin (Life Technologies) at 37 °C in an atmosphere of 5 % CO 2 in air and were handled as described in .

Techniques: Microscopy, Amplification, Control, Incubation, Concentration Assay

Journal: Molecules

Article Title: Imidazole Carbamates as a Promising Alternative for Treating Trichomoniasis: In Vitro Effects on the Growth and Gene Expression of Trichomonas vaginalis

doi: 10.3390/molecules29112585

Figure Lengend Snippet: Cytotoxic effects of AGR compounds and the commercial drugs MTZ and NTZ on Caco-2 and HT29 cells. Concentration–response curves for the viability of ( A ) Caco-2 and ( B ) HT29 cells treated with increasing concentrations of AGR compounds. Concentration–response curves for the viability of ( C ) Caco-2 and ( D ) HT29 cells treated with increasing concentrations of MTZ and NTZ. Cell viability was determined using trypan blue dye exclusion (0.4%) (1:1, v / v ) in a Neubauer chamber, as well as an XTT proliferation assay. Values represent the mean ± standard deviation of three independent experiments.

Article Snippet: The Caco-2 and HT29 cell lines were provided by Luz Maria Rocha Ramirez from Hospital Infantil de México Federico Gómez, Secretaría de Salud.

Techniques: Concentration Assay, Proliferation Assay, Standard Deviation

Journal: Molecules

Article Title: Imidazole Carbamates as a Promising Alternative for Treating Trichomoniasis: In Vitro Effects on the Growth and Gene Expression of Trichomonas vaginalis

doi: 10.3390/molecules29112585

Figure Lengend Snippet: In vitro trichomonacidal activity and cytotoxicity of the AGR compounds.

Article Snippet: The Caco-2 and HT29 cell lines were provided by Luz Maria Rocha Ramirez from Hospital Infantil de México Federico Gómez, Secretaría de Salud.

Techniques: In Vitro, Activity Assay